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Objective: To study the effect of VEGF antisense oligodeoxynucleotide (VEGF ASODN) on VEGF expression in acute monocyte leukemic cell line U937 in vitro. Methods: U937 cells were incubated with VEGF ASODN (final concentration as follows: 10, 20 and 30 μmol/L respectively) or scrambled sequence, compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR, VEGF protein was measured by Weste blot. Results: VEGF ASODN obviously inhibited expression of VEGF mRNA in U937 cell, compared with scrambled sequence and negative control(P＜0.05). And the inhibition effect was most remarkable after 24 h, which is related with the dose of VEGF ASODN (P＜0.05). Scrambled sequence groups had no significant difference compared with negative control groups (P＞0.05). VEGF ASODN obviously inhibited expression of VEGF protein, compared with scrambled sequence and negative control (P＜0.05). Conclusion: The expressions of VEGF at mRNA and protein levels in leukemic cell line U937 are down-regulated after being treated with VEGF ASODN.