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目的研究组蛋白乙酰化转移酶亚型类固醇受体共激活因子1(SRC1)在小鼠心脏中的时空表达规律,初步探讨其与心脏发生发育的关系。方法取胎龄E7.5～18、出生1d和3个月成年小鼠正常心脏,每个时间点选9个标本,采用免疫组织化学SP法观察SRC1蛋白在不同发育阶段心脏的空间表达;每个时间点选6组标本,利用蛋白质印迹分析技术检测其在小鼠心脏发育过程中时序性表达变化。结果免疫组织化学结果显示,SRC1蛋白在E7.5胚胎心脏原基中不表达;E8.5～E9.5心管中微弱表达;E10.5后心脏发育各时期,小梁弱表达,心脏其他区域均有较强而广泛表达。蛋白质印迹分析结果显示,E10.5以后的心脏发育阶段,SRC1蛋白在E10.5较低表达,与E11.5、E12.5相比表达差异有统计学意义(P<0.05),与其他发育时间点相比表达差异无统计学意义(P>0.05);E11.5～E12.5达到表达高峰,此两个发育时间点相比表达差异无统计学意义(P>0.05),与其他发育时间点相比表达差异有统计学意义(P<0.05);此后表达下降并持续低表达至成年鼠期,此阶段表达差异无统计学意义(P>0.05)。结论 SRC1蛋白广泛表达于E8.5后的发育心脏,并呈时空动态变化,提示其参与了心脏发育的整体调控,可能与心脏间隔早期的诱导形成关系更为密切。
Objective To study the spatial and temporal expression pattern of histone deacetylase (HSC) coactivator 1 (SRC1) in mouse hearts and to explore its relationship with cardiac development. Methods The normal heart of adult mice aged from E7.5 to E18 and birth to 1 and 3 months were selected. Nine samples were selected at each time point. The expression of SRC1 protein in different developmental stages was observed by immunohistochemical SP method. Six time points were selected six groups of samples, Western blot analysis using the technology to detect changes in the mouse heart during the development of temporal changes. Results Immunohistochemical results showed that SRC1 protein was not expressed in embryonic heart of E7.5 embryos; weakly expressed in E8.5 ~ E9.5 cardiac tubes; weakly expressed in trachea Areas are strong and widely expressed. Western blot analysis showed that the expression of SRC1 protein in E10.5 was lower than that in E10.5 after E10.5, and there was significant difference between E10.5 and E12.5 (P <0.05) (P> 0.05); E11.5 ~ E12.5 reached the peak of expression, there was no significant difference between the two developmental time points (P> 0.05), but the difference was not statistically significant (P <0.05). After that, the expression decreased and continued to be low in adult mice. There was no significant difference in the expression between these two groups (P> 0.05). Conclusions SRC1 protein is widely expressed in the developmental heart after E8.5 and shows spatiotemporal dynamics, suggesting that it is involved in the overall regulation of cardiac development and may be closely related to the early induction of cardiac interval.