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【目的】探讨~(60)Co-γ射线对2个不同月季品种扦插苗的辐射诱变效应,确定其辐射处理的半致死剂量,为月季的辐射育种提供理论基础。【方法】以2个月季品种“绯扇”、“卡罗拉”的扦插苗为材料,采用5个剂量的~(60)Co-γ射线进行辐射处理,分析比较不同剂量下的成活率、死亡率以及植株生长、变异情况,明确辐射剂量与死亡率之间的关系,并确定半致死剂量。【结果】随着辐射剂量的增加,成活率逐渐降低,死亡率逐渐增加,辐射剂量与死亡率之间呈正相关;“绯扇”的半致死剂量为71 Gy、“卡罗拉”的半致死剂量为68 Gy;辐射剂量越大辐射效应越显著;植株变异为性状变异,表现为叶片和花朵的变异。【结论】不同月季品种对~(60)Co-γ射线辐射的敏感性不同,辐射剂量越大辐射效应越显著,不同月季品种的半致死辐射剂量存在差异。
【Objective】 The purpose of this study was to investigate the radiation induced effects of ~ (60) Co-γ-rays on cutting seedlings of two different rose varieties and to determine the semi-lethal dose of radiation treatment, providing the theoretical basis for radiation breeding of rose. 【Method】 The cuttings of two “Scallop” and “Corolla” cultivars were irradiated with ~ (60) Co-γ rays at 5 doses for the analysis of survival at different doses Rate, mortality and plant growth, variation, clear radiation dose and mortality relationship, and to determine the semi-lethal dose. 【Result】 With the increase of radiation dose, the survival rate decreased gradually and the mortality rate increased gradually. There was a positive correlation between radiation dose and mortality rate. The median lethal dose of “Scorpion fan” was 71 Gy, and “Corolla” The lethal dose was 68 Gy. The greater the radiation dose was, the more significant the radiation effect was. The variation of plant was the variation of traits, which showed the variation of leaves and flowers. 【Conclusion】 Different varieties of rose have different sensitivity to ~ (60) Co-γ radiation. The higher the radiation dose, the more significant the radiation effect. The differences of the LD50 of different rose varieties are different.