The evolution of thermohaline structure at the upper ocean during three tropical cyclones (TCs) in the Northwest Pacific was studied in this study based on successive observation by two new-style underwater gliders during fall 2018. These remote-controllable gliders with CTD sensor enabled us to explore high frequency responses of temperature, salinity, mixed and barrier layers in the upper ocean to severe TCs in this area. Results showed that three significant cooling-to-warming and stratification destructing-to-reconstructing processes at the mixed layer occurred during the lives of three TCs. The maximal cooling of SST all reached ≥0.5℃ although TCs with different intensities had different minimal distances to the observed area. Under potential impacts of solar radiation, tide and inertial motions, the mixed layer depth possessed significant high-frequency fluctuations during TC periods. In addition, barrier layers appeared and vanished quickly during TCs, accompanied with varied temperature inversion processes.