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目的探讨新生儿同种免疫性血小板减少症(NAIT,Neonatal Allo-immune Thrombocytopenic)的试验诊断方法。方法采用常规凝集试验进行红细胞血型系统相关检测,采用MASPAT试剂盒单克隆抗体固相血小板抗体实验进行血小板特异性抗体鉴定,采用酶联免疫(ELISA)方法进行HLA-Ⅰ类抗体检测,同时用PCR-SSP方法进行被检者HPA基因分型。结果虽然母亲为RhD(-)且既往有多次妊娠史,但无Rh系新生儿溶血病血清学表现,母亲HPA基因型为HPA15a/15a,父亲和患儿基因型均为HPA15a/15b。相关试验证实既有HLA-Ⅰ类抗体,也有血小板特异性体抗HPA-15b。结论按照本系列试验方案进行检测,能够对引致NAIT的血小板抗体进行筛查分类,并为NAIT的科学准确诊断及治疗提供依据。
Objective To investigate the diagnostic methods of Neonatal Allo-immune Thrombocytopenic (NAIT). Methods Routine agglutination test was used to detect the erythrocyte blood group system. MASPAT kit was used to detect platelet-specific antibodies by monoclonal antibody against platelet-specific antibody. HLA-Ⅰ antibody was detected by enzyme-linked immunosorbent assay (ELISA) -SSP method was tested HPA genotyping. Results Although the mother was RhD (-) and had multiple pregnancies in the past, there was no serological manifestations of Rh hemolytic disease. The genotypes of HPA15a / 15a and HPA15a / 15b were both HPA15a and 15a. Related tests confirmed that both HLA-I antibodies, but also platelet-specific antibodies against HPA-15b. Conclusions According to this series of test programs for testing, can cause NAIT-induced platelet antibodies were screened for classification and NAIT for scientific and accurate diagnosis and treatment provide the basis.