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目的 从本实验室已建立的 Ig G类抗日本血吸虫抗原不同表位的单抗中筛选抗独特型抗体 NP30的独特型抗体 (id,Ab1 )。方法 应用间接 EL ISA经典法 ,NP30作为抗原包板。加 Ig G各亚类杂交瘤细胞培养上清孵育 ,用辣根过氧化物酶 (HRP)标记抗鼠 Ig G捕捉。结果 单抗 NP5 6与NP30呈阳性反应 ,NP5 6的 Ig同型为 Ig G2 b。免疫组织化学显示 NP5 6与虫卵内毛蚴头腺及毛蚴膜呈阳性反应。结论 NP5 6是 NP30的特异性 id,可用于 NP30模拟抗原的鉴定
Objective To screen the idiotypic antibodies (id, Ab1) of the anti-idiotypic antibody NP30 from the monoclonal antibodies against different epitopes of IgG antigens of Schistosoma japonicum in our laboratory. Methods Indirect EL ISA classical method, NP30 as antigen package board. Plus Ig G hybridoma cell culture supernatants were incubated with horseradish peroxidase (HRP) labeled anti-mouse Ig G capture. Results The McAbs NP5 6 and NP30 were positive. The Ig isotype of NP5 6 was Ig G2 b. Immunohistochemistry showed that NP5 6 showed positive reaction with the spleen and metacercariae of E. coli. Conclusion NP5 6 is a specific id of NP30 and can be used for the identification of NP30 mimic antigen