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目的探讨Twist和Akt2蛋白在肾透明细胞癌(RCCC)组织中的表达及其与RCCC临床病理特征的关系。方法采用免疫组化Eli Vision TM Plus法检测43例RCCC及20例癌旁正常肾组织中Twist和Akt2蛋白的表达,并分析二者表达与临床病理特征的关系。结果 RCCC和癌旁正常肾组织中的Twist蛋白阳性表达率分别为67.44%和20.00%,具有显著差异(P<0.01);Twist蛋白表达与RCCC病理分级、局部淋巴结转移呈显著正相关(P<0.05)。RCCC和癌旁正常肾组织中的Akt2蛋白阳性表达率分别为60.47%、15.00%,差异有统计学意义(P<0.01);Akt2蛋白表达与RCCC病理分级、局部淋巴结转移呈显著正相关(P<0.01;P<0.05)。Twist和Akt2蛋白的表达与患者性别、年龄、肿瘤大小无关(P>0.05)。Twist和Akt2蛋白表达呈显著正相关(P<0.05)。结论 Twist和Akt2蛋白过表达与RCCC发生发展相关,二者可能存在协同作用。
Objective To investigate the expression of Twist and Akt2 proteins in renal clear cell carcinoma (RCCC) and its relationship with the clinicopathological features of RCCC. Methods The expressions of Twist and Akt2 protein in 43 cases of RCCC and 20 cases of adjacent normal renal tissues were detected by immunohistochemical Eli Vision TM Plus method. The relationship between them and clinicopathological features was analyzed. Results The positive rates of Twist protein in RCCC and adjacent normal renal tissues were 67.44% and 20.00%, respectively (P <0.01). The expression of Twist protein was positively correlated with the pathological grade of RCCC and the local lymph node metastasis (P < 0.05). The positive rates of Akt2 protein in RCCC and adjacent normal renal tissues were 60.47% and 15.00%, respectively (P <0.01). There was a significant positive correlation between Akt2 protein expression and pathological grade and lymph node metastasis <0.01; P <0.05). The expression of Twist and Akt2 protein had no relation with patient’s sex, age and tumor size (P> 0.05). Twist and Akt2 protein expression was significantly correlated (P <0.05). Conclusion The overexpression of Twist and Akt2 is associated with the occurrence and development of RCCC, and they may have synergistic effects.