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目的：研究不同剂量碱性成纤维细胞生长因子（ｂＦＧＦ）对人胚成骨样细胞ＤＮＡ、胶原蛋白和碱性磷酸酶合成的影响。方法：实验分为４组，每组６孔，每组分别加入浓度为０．１ｎｇ／ｍｌ，１ｎｇ／ｍｌ，１０ｎｇ／ｍｌ，１００ｎｇ／ｍｌ的ｂＦＧＦ，每组均设空白对照，在实验后第１，３，５，７天分别检测人胚成骨样细胞３Ｈ－ＴｄＲ和３Ｈ－脯氨酸的掺入量，以及碱性磷酸酶合成量。结果：ｂＦＧＦ可明显促进人胚成骨样细胞的ＤＮＡ合成，但却抑制其胶原蛋白和碱性磷酸酶的合成，ｂＦＧＦ对人胚成骨样细胞具有双重效应。在含量为０．１～１０ｎｇ／ｍｌ，呈剂量依赖性，１０ｎｇ／ｍｌ时ｂＦＧＦ的作用最强。结论：ｂＦＧＦ在骨形成中有一定作用，但仍需与其他细胞生长因子协同作用
OBJECTIVE: To study the effects of basic fibroblast growth factor (bFGF) on the DNA, collagen and alkaline phosphatase synthesis of human osteoblast-like cells. Methods: The experiment was divided into 4 groups with 6 wells in each group. The bFGF with the concentration of 0.1ng / ml, 1ng / ml, 10ng / ml and 100ng / ml were added in each group. Blank control was set in each group. 3H-TdR and 3H-proline incorporation, and alkaline phosphatase synthesis of human osteoblast-like cells were detected on days 1, 3, 5 and 7, respectively. Results: bFGF promoted the DNA synthesis of human osteoblast-like cells, but inhibited the synthesis of collagen and alkaline phosphatase. BFGF had a dual effect on human osteoblast-like cells. In the dosage of 0.1 ~ 10ng / ml, in a dose-dependent, 10ng / ml bFGF strongest role. Conclusion: bFGF plays a role in bone formation, but it still needs to cooperate with other cell growth factors