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目的:探讨大鼠肝大部切除再生过程中肝和垂体内纤维粘连蛋白变化变化.方法:用免疫组织化学法观察大鼠肝大部切除术后0.5天1天、1.5天、2天、3天、7天时,肝内和垂体远测部滤泡垦状细胞细胞纤维粘连蛋白的变化,并用图像分析仪进行定量测定.结果:肝大部切除术后1.5天,肝内纤维粘连蛋白阳性染色增强,其平均光密度高于对照组(P<0.05);术后2～3天,镜下可见纤维粘连蛋白染色呈强阳性,尤其在肝组织增生部位,变化十分明显,至术后第3天达高峰,平均光密度明显增高,为正常组的1.6倍(P<0.01)术后1.5～77天纤维粘连蛋白阳性包的肝细胞数增多,反应增强,以术后1.5天时最为明显,并可见较多的双核细胞里强阳性反应.肝大部切除术后2～3天垂体远侧部纤维粘连蛋白阳性FS细胞增多,反应也增强.结论:在肝大部切除后机体可能通过表达纤维粘连蛋白从整体及局部调节肝的再生.
Objective: To investigate the changes of fibronectin in liver and pituitary during the process of hepatic resection in rats.Methods: Immunohistochemistry was used to observe the changes of fibronectin in the liver after 1, 1.5, 2 and 3 days Day and 7 days, the changes of fibronectin in follicular cells in the intrahepatic and pituitary gland were quantified by image analyzer.Results: The positive rate of intrahepatic fibronectin staining was 1.5 days after subtotal hepatectomy (P <0.05); 2 ~ 3 days after operation, the staining of fibronectin was strongly positive, especially in the liver tissue hyperplasia, the change is very obvious, to 3 The peak of Tianda, the average optical density was significantly higher than the normal group 1.6 times (P <0.01) 1.5 to 77 days postoperative fibronectin positive package increased the number of liver cells, the reaction was enhanced to 1.5 days after the most obvious and More positive positive cells could be seen in the binuclear cells.Focal fibronectin positive FS cells increased in the distal part of the pituitary gland 2 to 3 days after partial hepatectomy.Conclusions: Laminin regulates liver regeneration overall and locally .