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应用食蟹猴疟原虫与体外培养人恶性疟原虫两种抗原作间接荧光抗体试验以检测疟疾抗体,结果居民146人中检出率分别为52.05％和53.42％,小学生107人检出率分别为18.69％和19.62％,阳性GMRT居民分别为45.60和58.09,小学生分别为42.87和53.84,后者较前者为高。在恶性疟流行区用恶性疟抗原测抗体更能反应当地疟疾流行状况。同时对羊抗人IgG和兔抗人IgG进行了对比,当工作浓度为1:16时,两种IgG均显示较高的灵敏性,将兔抗人IgG浓度稀释至1:32
Application of malaria cynomolgus and in vitro culture of human Plasmodium falciparum two antigens indirect fluorescent antibody test to detect malaria antibodies, the results of 146 residents were detected in 52.05% and 53.42%, respectively, the detection rate of 107 pupils were 18.69% and 19.62% respectively. The positive GMRT residents were 45.60 and 58.09 respectively, while the pupils were 42.87 and 53.84 respectively, the latter being higher than the former. In the endemic area of falciparum malaria, anti-malarial antigens can be used to better reflect the prevalence of malaria. At the same time, goat anti-human IgG and rabbit anti-human IgG were compared. When the working concentration was 1:16, both IgGs showed high sensitivity. The rabbit anti-human IgG concentration was diluted to 1:32