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选取60只5~6周龄的雄性Wistar大鼠,随机分为5组:对照组、染氟组、维生素E(Vit E)干预组、硒干预组、Vit E+硒联合干预组。灌胃12周后测量各组大鼠血清氟、尿氟含量及血清总碱性磷酸酶(ALP)活性,并测量胫骨长度、横径和重量及骨密度(BMD)。与对照组比较,染氟组大鼠血清氟、尿氟含量和血清ALT活性显著增高;全身骨骼和胫骨BMD降低;胫骨长度、横径减小,重量减轻。与染氟组比较,单独给予Vit E干预,大鼠血清氟、尿氟含量和血清ALT活性下降。单独硒干预组、Vit E+硒联合干预组大鼠血清氟、尿氟含量和血清ALP活性下降;全身骨骼和胫骨BMD增高;胫骨长度增长、重量增加。与Vit E和硒单独干预组相比,Vit E+硒联合干预组上述测定指标改善更为明显。提示Vit E+硒联合干预对过量氟所致骨损伤有一定的保护作用。
Sixty male Wistar rats aged 5-6 weeks were randomly divided into five groups: control group, fluoride group, Vit E intervention group, selenium intervention group and Vit E + selenium intervention group. After 12 weeks of gavage, serum fluoride and urinary fluoride contents and total alkaline phosphatase (ALP) activity were measured in each group, and the length, transverse diameter, weight and bone mineral density (BMD) of tibia were measured. Compared with the control group, serum fluoride, urinary fluoride and serum ALT activity in the fluoridated rats were significantly increased; the BMD of the whole body and the tibia were decreased; the length and diameter of the tibia were reduced, and the weight was reduced. Compared with the fluoridated group, Vit E alone was administered to the rats, and serum fluoride and urinary fluoride contents and serum ALT activity were decreased. Selenium intervention group, Vit E + selenium intervention group serum fluoride, urinary fluoride and serum ALP activity decreased; body bones and tibia BMD increased; tibia length increased, weight increased. Compared with Vit E and selenium alone intervention group, Vit E + selenium intervention group improved more obviously. Prompt vit E + selenium intervention on excessive fluoride-induced bone injury have a protective effect.