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目的:应用正电子发射断层扫描(positron emission tomography,PET)/计算机断层成像(computed tomography,CT)技术研究棕色脂肪组织(brown adipose tissue,BAT)活动情况对荷瘤小鼠肿瘤组织摄取18F-氟-2-脱氧葡萄糖(~(18)F-luoro-2-deoxyglucose,~(18)F-FDG)的影响。方法:建立人肺癌NCI-H1299小鼠移植瘤模型,随后采用人工控制实验条件的方法调节小鼠BAT的活动;实验分为2组:抑制组通过禁食、麻醉和加热处理抑制BAT的活动,激发组则通过食物干预和冷刺激激发BAT的活动。对2组小鼠进行micro-PET/CT成像检测,比较BAT不同活动状态下小鼠肿瘤摄取18F-FDG的情况,并计算标准摄取值(standard uptake value,SUV)。结果:BAT激发组小鼠的肿瘤在PET/CT图像中几乎是不可见的,其摄取18F-FDG的SUV值为0.15±0.06,显著低于BAT抑制组的0.58±0.20(P<0.05)。结论:BAT活动的增加会显著降低肿瘤对~(18)F-FDG的摄取,从而影响肿瘤的成像效果。
OBJECTIVE: To study the effect of brown adipose tissue (BAT) activity on the tumor tissues of tumor-bearing mice by using positron emission tomography (PET) / computed tomography (CT) -2-deoxyglucose (~ (18) F-luoro-2-deoxyglucose, ~ (18) F-FDG) METHODS: Human lung cancer NCI-H1299 xenograft model was established. Then the activity of BAT was regulated by artificial control of experimental conditions. The experiment was divided into two groups: the inhibition group inhibited the activity of BAT through fasting, anesthesia and heat treatment, The challenge group stimulated BAT activity through food intervention and cold stimulation. The micro-PET / CT imaging of the two groups of mice was performed. The tumor uptake of 18F-FDG in different BAT states was compared and the standard uptake value (SUV) was calculated. Results: Tumors in BAT-challenged mice were almost invisible in PET / CT images. The SUV of uptake of 18F-FDG was 0.15 ± 0.06, significantly lower than that of BAT inhibition group (0.58 ± 0.20, P <0.05). Conclusion: The increase of BAT activity can significantly reduce the uptake of 18F-FDG in the tumor and thus affect the tumor imaging.