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应用香蕉束顶病毒(BBTV)的单克隆抗体(McAb)建立了检测香蕉束顶病的间接ELISA方法,其检测香蕉病组织汁液的最大稀释度为1:1280,健康香蕉组织汁液无非特异性反应。用间接ELISA方法检测了采自广东、广西9个市(县)的香蕉产区的80个大田香蕉束顶病样本和25个荫棚香蕉试管苗样本,其中大田样本BBTV的带毒率为76.5％,试管苗样本的带毒率为8.0％。香蕉束顶病株不同部位BBTV含量检测表明,BBTV在病株中系统性分布,叶肉和中脉中的BBTV含量无差异。下部老叶与上部嫩叶相比,症状较轻,病毒含量也较低。
An indirect ELISA method was developed for the detection of banana bunchy top disease using the monoclonal antibody (McAb) of banana bunchy top virus (BBTV). The maximum dilution of banana juice was 1: 1280, and the non-specific reaction of healthy banana juice . Eighty wild banana bunchy top samples and 25 shade banana in vitro samples collected from banana producing areas in nine cities of Guangdong and Guangxi were detected by indirect ELISA. The infection rate of BBTV in field samples was 76.5 %, Test-tube sample of the virulence rate of 8.0%. The detection of BBTV content in different parts of the banana bunchy top strain showed that BBTV was systematically distributed in the diseased plants and there was no difference in BBTV content in the mesophyll and midrib. The lower part of the old leaves compared with the upper leaves, the symptoms are mild, the virus content is also low.