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目的以知母种子为试验材料,对知母的组织培养进行了初步研究,以期建立知母的再生体系。方法采用植物组织培养和单因子试验的方法对知母无菌体系的建立、分蘖芽的增殖、分蘖愈伤组织的诱导和再分化以及再生苗的移栽进行研究。结果 知母种子最佳的消毒方式是75%酒精处理30 s后用0.1%HgCl2处理15 min;知母分蘖芽增殖的最佳培养基是MS+KT 1 mg/L+NAA 0.5 mg/L;知母分蘖愈伤组织诱导的最佳培养基是MS+KT 2 mg/L+NAA 0.5 mg/L;知母愈伤组织再分化的最佳培养基是MS+KT 2 mg/L+NAA 0.1 mg/L;知母愈伤组织再生芽最佳的生根培养基是1/2MS+NAA 0.5 mg/L;知母试管苗最佳的移栽基质是腐殖土。结论本实验建立了知母的再生体系,为知母试管苗的工厂化生产奠定了技术基础。
Aim To study the tissue culture of Anemarrhena asphodeloides with the help of Anemarrhena asphodeloides, with a view to establishing a regeneration system. Methods The establishment of aseptic system, the proliferation of tiller buds, the induction and re-differentiation of tillering callus and the transplanting of regenerated seedlings were studied by plant tissue culture and single factor test. Results The best disinfection method for A. annua seed was 75% alcohol for 30 s and 0.1% HgCl2 for 15 min. The best medium for the proliferation of Anemarrhena asphodeloides was MS + KT 1 mg / L + NAA 0.5 mg / L; The optimal culture medium for inducing tillering callus of A. fortunei was MS + KT 2 mg / L + NAA 0.5 mg / L. The best medium for callus re-differentiation was MS + KT 2 mg / L + NAA 0.1 mg / L. The best rooting medium of regenerated buds of Anemarrhena asphodeloides was 1 / 2MS + NAA 0.5 mg / L. The best transplanting medium for Anemarrhena astrology plantlets was humus soil. Conclusion This experiment established the regeneration system of Anemarrhena asphodeloides, which laid the technical foundation for the factory production of Anemarrhena asphodeloides plantlets.