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目的探讨糖尿病早期肾损害诊断指标。方法对 5 0例正常对照组和 5 0例糖尿病患者采用终点法测定尿N 乙酰 β D 氨基葡萄糖苷酶 (NAG) ,ELISA方法检测尿视黄醇结合蛋白 (RBP)、微量白蛋白 (mALB)和尿转铁蛋白 (TRF) ,比色法测尿肌酐。结果正常对照组尿NAG/Cr为 (0 .5 2± 0 .4 6 )U/mmol,RBP/Cr为 (7.4 6± 5 .0 2 ) μg/mmol,mALB为(3.6 1± 2 .4 6 )mg/L ,TRF/Cr为(0 .0 5± 0 .0 3)mg/mmol;糖尿病患者尿NAG/Cr、RBP/Cr、mALB及TRF/Cr值较正常对照组增高 (P <0 .0 1) ;两项指标联合检测 ,其阳性率分别为 :NAG +mALB 86 % ,NAG +TRF 88% ,NAG +RBP 90 %。结论联合检测尿NAG与RBP是诊断糖尿病早期肾损害的最灵敏与可靠的实验室指标。
Objective To investigate the diagnostic indicators of early diabetic nephropathy. Methods Urinary N acetyl beta D glucosaminidase (NAG) was measured by the end point method in 50 normal controls and 50 diabetic patients. Urinary retinol binding protein (RBP), microalbumin (mALB) And urinary transferrin (TRF), urine creatinine colorimetry. Results The urinary NAG / Cr in the normal control group was (0.52 ± 0.46) U / mmol, the RBP / Cr was (7.46 ± 5.22) μg / mmol and the mALB was (3.6 ± 2. Cr, TRP / Cr was (0. 05 ± 0. 03) mg / mmol. The urinary NAG / Cr, RBP / Cr, mALB and TRF / Cr in urine of diabetic patients were significantly higher than those of normal controls (P < 0 .0 1). The positive rates of the two indexes were 86% for NAG + mALB, 88% for NAG + TRF and 90% for NAG + RBP. Conclusions The combined detection of urinary NAG and RBP is the most sensitive and reliable laboratory index for diagnosing early diabetic nephropathy.