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目的比较研究HLA-Ⅰ、Ⅱ血清学分型与基因分型结果,分析HLA-A、B、DR血清学分型误定规律,提高移植配型的准确性。方法应用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对240名骨髓资料库中已用血清学分型的自愿者进行HLA-A、B、DR基因分型,并对血清学分型与基因分型结果进行低分辨水平的比较研究。结果HLA-A特异性血清学分型错误率在纯合子与杂合子中分别占30.65%与11.52%,总错误率14.35%;HLA-B特异性血清学分型错误率在纯合子与杂合子中分别占42.22%与16.15%,总错误率18.85%;HLA-DR特异性血清学分型错误率在纯合子与杂合子中分别占37.50%与14.58%,总错误率18.63%。HLA-A特异性血清学分型假阴性16.55%,假阳性1.44%,错误指认特异性7.42%,HLA-B分别为20.32%,1.84%和13.56%,HLA-DR分别为13.33%,2.21%和10.05%。结论HLA-A、B、DR纯合子血清学分型错误率明显高于对应的杂合子分型,HLA-B,DR特异性血清学分型错误率显著高于HLA-A特异性;为了提高移植配型的准确性,骨髓资料库中自愿者HLA-A、B、DR位点须重新用基因分型方法分型。
Objective To compare the serological typing and genotyping results of HLA-Ⅰ and Ⅱ, and to analyze the misclassification rules of HLA-A, B and DR serological typing and to improve the accuracy of transplantation matching. Methods The genotypes of HLA-A, B, and DR were determined by polymerase chain reaction-sequence-specific primers (PCR-SSP) in 240 bone marrow donors with serological typing and serological typing And genotyping results for low-resolution comparative study. Results The HLA-A specific serotyping error rates were 30.65% and 11.52% respectively in homozygotes and heterozygotes, with a total error rate of 14.35%. HLA-B-specific serotyping error rates were between homozygous and heterozygous Accounting for 42.22% and 16.15% respectively, with a total error rate of 18.85%. The HLA-DR-specific serotyping error rates were 37.50% and 14.58% respectively for homozygotes and heterozygotes, with a total error rate of 18.63%. The HLA-A-specific serotypes were 16.55% false negative, 1.44% false positive, 7.42% false positive, HLA-B was 20.32%, 1.84% and 13.56% respectively, HLA-DR was 13.33% and 2.21% 10.05%. Conclusion The serological typing error rate of HLA-A, B and DR homozygotes was significantly higher than that of the corresponding heterozygote. HLA-B and DR-specific serotyping error rates were significantly higher than HLA-A specificity. Type of accuracy, the HLA-A, B, DR sites of volunteers in the bone marrow database should be re-genotyped by genotyping method.