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目的观察复方当归贝母苦参气雾和灌胃给药方式对吸烟致慢阻肺模型小鼠的影响,对其药效进行评价,并对其作用机制进行剖析讨论。方法收集了肺泡灌洗液(BALF)和肺组织,BALF进行细胞分类计数及细胞因子测定。取肺组织做组织切片进行HE染色,观察炎症细胞状况。取左上肺提取mRNA进行KC、Muc5AC的检测。结果与模型组对比,复方灌胃和气雾组均能减轻肺组织病理切片上的炎性细胞浸润,其中气雾组较灌胃组缓减轻效果更明显;与模型组相比,灌胃和气雾给药均能显著降低KC、MIP-2含量及Muc5AC的mRNA表达;另外气雾组还可显著降低TNF-?含量及KC的mRNA。结论复方当归贝母苦参气雾给药较灌胃给药对吸烟模型小鼠的肺部炎症反应、黏液分泌具有更强的抑制作用,其作用机制可能与气雾给药降低TNF-a的含量及KC的mRNA表达更明显有关。
Objective To observe the effects of Fufang Angelica Coptidis-Radix Sophorae Flavescens and intragastric administration on mice with chronic obstructive pulmonary disease (COPD), and to evaluate its pharmacodynamics. The mechanism of action is discussed and discussed. Methods BALF, lung and BALF were collected for cell counting and counting of cytokines. Take lung tissue tissue sections for HE staining to observe the status of inflammatory cells. Take the left upper lung extract mRNA for KC, Muc5AC detection. Results Compared with the model group, compound gavage and aerosol groups could both reduce the inflammatory cell infiltration on the pathological section of lung tissue, and the effect of relieving light was more obvious in the aerosol group than in the gavage group. Compared with the model group, Administration can significantly reduce the KC, MIP-2 content and mRNA expression of Muc5AC; the other aerosol group can significantly reduce TNF-α content and KC mRNA. Conclusions Fufang Angelica Coptidis Radix Ophiopogon japonicus aerosol can significantly inhibit lung inflammatory response and mucus secretion in mice with smoking, and its mechanism may be related to the decrease of TNF-α Content and KC mRNA expression more significantly related.