CD23分子又称为低亲合力IgE FC受体(FcERⅡ),分布广泛,其在细胞膜表面的表达与机体免疫应答及病理过程都存在显著关系。因此建立有效的膜CD 2_3分子的检测方法对于开展CD 23分子的研究及临床诊断均有十分重要的意义。由于单克隆抗体获取困难,我们根据其能特异性地与IgE相结合的特点,建立了IgE介导的间接荧光法,以用于膜CD23分子的分析。 CD23 molecule is also called as low affinity IgE Fc receptor (FcERII), widely distributed. The expression of CD23 on the surface of cell membrane has a significant relationship with immune response and pathological process. Therefore, the establishment of an effective detection method of CD 2 3 molecules is very important for the research and clinical diagnosis of CD 23. Due to difficulties in obtaining monoclonal antibodies, IgE-mediated indirect fluorescence based on its ability to specifically bind to IgE was developed for the analysis of membrane CD23 molecules.