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It is essential to predict the treatment efficacy of pancreatic carcinoma early.The purpose of this study was to examine whether ~(18)F-FDG(2’-deoxy-2’-[~(18)F]fiuoro-D-glucose) or ~(18)F-FLT(3’-deoxy-3’-~(18)F-fluorothymidine) PET can be used for chemosensitivity testing by investigating the binding characteristic of ~(18)F-FDG or ~(18)F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ~(18)F-FDG or ~(18)F-FLT on Patu 8988.Under the conditions of 1×10~6 cells,3.7 kBq ~(18)F-FDG or ~(18)F-FLT,and incubation at 37℃for 100 min,the cell uptake of ~(18)F-FDG and ~(18)F-FLT was(60.60±3.05)%and(50.57±2.81)%,respectively.There was a significant decrease in TKl-LI(thymidine kinase 1 labeling index) 24 h after administration of gemcitabine.The uptakes of ~(18)F-FDG and ~(18)F-FLT were negatively correlated with the doses of gemcitabine(r= -0.928 for ~(18)F-FDG,r= -0.876 for ~(18)F-FLT,P<0.01).When same doses of gemcitabine were administered,the ~(18)F-FLT uptake inhibition rate was significantly higher than that of ~(18)F-FDG(P<0.01).These results indicate that the response to gemcitabine could be predicted as early as 24 h by ~(18)F-FDG or ~(18)F-FLT PET scans.~(18)F-FLT is more sensitive than ~(18)F-FDG to predict the response to therapy.
It is essential to predict the treatment efficacy of pancreatic carcinoma early. The purpose of this study was to examine whether ~ (18) F-FDG (2’-deoxy-2’- [18 F] fiuoro- (18) F-FLT (3’-deoxy-3’- (18) F-fluorothymidine) PET can be used for chemosensitivity testing by investigating the binding characteristic of ~ ) F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ~ (18) F-FDG or ~ (18) F-FLT on Patu 8988.Under the conditions of 1 × 10 ~ 6 cells, (18) F-FDG and ~ (18) F-FLT was incubated at 37 ℃ for 100 min, and the cell uptake of ~ 3.05)% and (50.57 ± 2.81)%, respectively. There was a significant decrease in TKl-LI (thymidine kinase 1 labeling index) 24 h after administration of gemcitabine.The uptakes of ~ ) F-FLT were negatively correlated with the doses of gemcitabine (r = -0.928 for ~ (18) F-FDG, r = -0.876 for ~ (18) F- FLT, P <0.01) administer The result of that response to gemcitabine could be predicted as early as 24 h by ~ (18) F-FLT uptake inhibition rate was significantly higher than that of ~ (18) F-FDG F-FDG or ~ (18) F-FLT PET scans. ~ (18) F-FLT is more sensitive than ~ (18) F-FDG to predict the response to therapy.