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利用由粳稻品种Asominori与籼稻品种IR2 4的杂交组合衍生的重组自交F1 0 家系 (RecombinantInbredLines,RIL)群体及其衍生的染色体片段置换系 (ChromosomeSegmentSubstitutionLines,CSSL)群体 ,进行了种子休眠性QTL的检测和遗传效应分析。其中CSSL群体有 2个 ,即CSSL1(以Asominori为背景 ,置换片段来自IR2 4)和CSSL2 (以IR2 4为背景 ,置换片段来自Asominori)。在RIL群体上共检测到 3个种子休眠性QTL ,分别位于第 3、6和 9染色体上 ;在CSSL1群体中检测到分布在第 1、3和 7染色体上的 3个休眠性QTL ;而在CSSL2群体上检测到的 3个QTL则分别位于第 1、2和 7染色体上。同时在两套CSSL群体上 ,分别检测到位于第 1、7染色体上位置相近且效应一致的休眠性QTL ,分析表明其所在的Asominori片段含对种子休眠性的增效基因 ,相应的IR2 4片段含有减效基因。
The QTLs for seed dormancy were detected using a population of recombinant inbred lines (RILs) derived from a cross between Asominori and indica variety IR2 4 and their populations derived from Chromosome Segment Subconfirmation Lines (CSSL) And genetic effects analysis. Among them, there are 2 CSSL groups, namely, CSSL1 (with Asominori as background and replacement fragment from IR2 4) and CSSL2 (with IR2 4 as background and substitution fragment from Asominori). Three seed dormancy QTLs were detected on RIL population, located on chromosomes 3, 6 and 9, respectively. Three dormant QTLs distributed on chromosomes 1, 3 and 7 were detected in CSSL1 population. The three QTLs detected on the CSSL2 population are located on chromosomes 1, 2 and 7, respectively. At the same time, two QTLs for QTLs on chromosome 1 and QTLs with similar effects on chromosome 1 and 2, respectively, were detected in the two CSSL populations. The results showed that the Asominori fragment in which QTLs were located contained the synergistic genes for seed dormancy, the corresponding IR2 4 fragment Contains the subtractive gene.