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在由1.0 mmol/Lβ-环糊精、乙二醇(1+9)和10 mmol/L硼砂组成的运行缓冲溶液(pH 9.11)中,采用22 kV的分离电压、25℃的毛细管柱温、200 nm的检测波长和5.0 s的压力(3450 Pa)进样时间,建立了可以在21 min内同时分离测定儿茶素、表儿茶素、原儿茶醛及原儿茶酸的高效毛细管电泳方法。检出限(S/N=3)依次为0.27、0.18、0.52和0.41μg/mL。方法用于普洱茶、丹参注射液和中药儿茶与金荞麦片中这4种组分的测定,相对标准偏差在4%以内,加标回收率在96.1%~105.4%之间。
In a running buffer (pH 9.11) composed of 1.0 mmol / L β-cyclodextrin, ethylene glycol (1 + 9) and 10 mmol / L borax, a separation voltage of 22 kV, a capillary column temperature of 25 ° C, 200 nm detection wavelength and 5.0 s pressure (3450 Pa) injection time to establish a simultaneous determination of catechin, epicatechin, protocatechuic aldehyde and protocatechuic acid in 21 min by high performance capillary electrophoresis method. The limits of detection (S / N = 3) were 0.27, 0.18, 0.52 and 0.41 μg / mL, respectively. The method was applied to the determination of four components in Pu’er tea, Salvia miltiorrhiza bunge and Crest tea and Chinese buckwheat. The relative standard deviations were less than 4%, and the recoveries were between 96.1% and 105.4%.