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目的研究豆腐果苷对慢性应激小鼠海马神经元再生的影响,探讨其抗抑郁作用的可能机制。方法给予小鼠多种慢性不可预知性应激刺激,建立小鼠抑郁模型,刺激前30 min给予豆腐果苷(10、20、40 mg.kg-1,ig),连续28 d。用免疫组化实验检测海马齿状回神经前体细胞分裂及脑源性神经营养因子(BDNF)表达,Western蛋白印迹法检测海马BDNF蛋白表达水平。结果免疫组化显示,慢性应激28 d小鼠海马齿状回神经前体细胞分裂减少,同时BDNF水平低下,均表现为阳性棕色颗粒缺失,豆腐果苷或盐酸氟西汀则明显增加神经前体细胞的分裂,并上调BDNF蛋白表达,提高BDNF水平。结论豆腐果苷对慢性应激小鼠的抗抑郁作用机制可能与促进海马齿状回神经前体细胞增殖,提高BDNF水平有关。
Objective To study the effect of tofu pectin on the regeneration of hippocampal neurons in chronic stress mice and to explore the possible mechanism of its antidepressant effect. Methods Various chronic unpredictable stress stimuli were given to mice. Depression models were established in mice. Tofuoside (10, 20, 40 mg.kg-1, ig) was administered 30 min before stimulation for 28 consecutive days. The hippocampal dentate gyrus neuron precursor cell division and expression of BDNF were detected by immunohistochemistry and the expression of BDNF protein in hippocampus was detected by Western blotting. Results Immunohistochemical staining showed that the division of neurons in dentate gyrus of hippocampus was decreased and the level of BDNF was low at the 28th day in chronic stress. Both of them showed the absence of positive brown granules, while tofuoside or fluoxetine hydrochloride significantly increased the number of neurons Somatic cell division, and upregulation of BDNF protein expression, increased BDNF levels. Conclusion The antidepressant mechanism of tofuoside in chronic stress mice may be related to promoting the proliferation of dentate gyrus neural precursor cells and increasing the level of BDNF in hippocampus.