用表面等离子体子共振生物传感器构建对心肌肌钙蛋白 I特异性的免疫传感器检测心肌肌钙蛋白 I,并建立两种检测方法 :直接法的最低检测限为 2 .5 μg/L,基于传感膜上的夹心免疫法的灵敏度为 0 .5 μg/L,检测范围为 0 .5～ 2 0 μg/L,批内及批间精密度分别为 3 .5 %～ 4.9% ,6.1 %～ 7.4% ;用夹心法及国外试剂盒对 40名健康献血者和 2 0例急性心肌梗死患者血清心肌肌钙蛋白 I水平进行检测 ,两者符合率为 95 % . Surface plasmon resonance biosensor was used to construct cardiac troponin I-specific immunosensor for detecting cardiac troponin I and two detection methods were established. The detection limit of direct method was 2.5 μg / L, Sensitivity of the sandwich immunoassay on the sensorial membrane was 0.5 μg / L, the detection range was 0.5-2.0 μg / L, the intra- and inter-assay precision was 3.5% -4.9%, 6.1% 7.4% respectively. Serum cardiac troponin I levels in 40 healthy blood donors and 20 acute myocardial infarction patients were detected by sandwich method and foreign kit, the coincidence rate was 95%.