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目的建立二十五味珍珠丸中人工牛黄胆酸的含量测定方法。方法采用RP-HPLC法进行人工牛黄中胆酸的含量测定,色谱柱:Kromasil C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-1%醋酸溶液(体积比为75∶25),流速:1.0 mL.min-1,柱温:40℃。采用蒸发光散射检测器,检测温度:40℃,灵敏度:10,氮气压力:1.0 bar。结果线性:0.47～3.96μg,相关系数r=0.999 6。平均回收率为100.3%(n=6),RSD=1.76%。结论该方法为评价和控制二十五味珍珠丸的质量提供了依据。
Objective To establish a method for the determination of artificial beef tallow cholic acid in Twenty-five pearl pills. Methods The content of bile acids in artificial bezoar was determined by RP-HPLC. The column was Kromasil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-1% acetic acid solution (volume ratio 75:25) Flow rate: 1.0 mL.min-1, Column temperature: 40 ° C. Using evaporative light scattering detector, detection temperature: 40 ℃, sensitivity: 10, nitrogen pressure: 1.0 bar. Results linear: 0.47 ~ 3.96μg, the correlation coefficient r = 0.999 6. The average recovery was 100.3% (n = 6), RSD = 1.76%. Conclusion This method provides the basis for the evaluation and control of the quality of Twenty-five pearl pills.