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目的对两种不同生物材料,纤维蛋白胶复合骨形态发生蛋白(FG-BMP)及重组合异种骨(RBX)对腱-骨界面愈合的效果进行比较评估。方法 51只新西兰大白兔,随机分为3组,行双侧前交叉韧带重建术,FG-BMP组腱-骨界面推注FG-BMP生物材料,RBX组则应用RBX生物材料,对照组则不施加任何材料。术后2、6、12周取材,行影像学检查、组织学分析及生物力学测试。结果 FG-BMP及RBX均能促进腱-骨间骨的生长。术后6周及12周,两组标本腱-骨间骨矿密度值(bone mineral dentisty,BMD)均明显高于对照组(P<0.05),其中术后6周时及术后12周,RBX组的BMD值均高于FG-BMP组;组织学结果显示,至术后12周,RBX组腱-骨界面显示移植肌腱周围被新生骨组织包绕,并且新生骨组织趋向移植肌腱方向生长,镜下新生骨组织数量明显大于FG-BMP组。生物力学测试结果显示,术后6周,应用注射型生物材料治疗的两组最大牵拉力都显著高于对照组,其中RBX组牵拉力最大(P<0.05);术后12周,RBX组明显高于对照组(P<0.05),FG-BMP组尽管高于对照组,但无显著性差异(P>0.05)。结论 FG-BMP组和RBX组均能促进腱-骨界面的愈合,但RBX在加速腱-骨界面愈合方面具有明显优势。
Objective To compare the effects of two different biomaterials, fibrin glue-bound bone morphogenetic protein (FG-BMP) and reconstituted xenogeneic bone (RBX) on tendon-bone interface healing. Methods Fifty-one New Zealand white rabbits were randomly divided into three groups. FG-BMP biomaterials were implanted into the tendon-bone interface of FG-BMP group, RBX biomaterials were used in RBX group, but not in control group Apply any material. At 2, 6, 12 weeks after operation, radiography, histological analysis and biomechanical tests were performed. Results Both FG-BMP and RBX promoted tendon-bone growth. At 6 weeks and 12 weeks after operation, the bone mineral dentisty (BMD) values of the two groups were significantly higher than those of the control group (P <0.05). At 6 weeks and 12 weeks after operation, The BMD of RBX group was higher than that of FG-BMP group. The histological results showed that the tendon-bone interface of RBX group was surrounded by new bone tissue around the tendon at 12 weeks after operation, and the new bone tissue tended to grow in the tendon , The number of newborn bone was significantly greater than the FG-BMP group. The results of biomechanical test showed that the maximum pulling force of the two groups treated with injectable biomaterials was significantly higher than that of the control group at 6 weeks after operation, of which RBX group had the largest pulling force (P <0.05); RBX Group was significantly higher than that of the control group (P <0.05). Although the FG-BMP group was higher than the control group, there was no significant difference (P> 0.05). Conclusion Both FG-BMP group and RBX group can promote tendon-bone interface healing, but RBX has obvious advantages in accelerating tendon-bone interface healing.