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背景:细胞凋亡是脑缺氧缺血神经细胞死亡的一种方式,体外试验表明神经生长因子(nervegrowthfactor,NGF)缺乏可诱导神经细胞凋亡。目的:探讨新生儿缺氧缺血性脑损伤(hypoxic-ischemicbraindamageHIBD)与细胞凋亡之间的关系,研究NGF对HIBD的保护作用,为用NGF治疗新生儿HIBD提供理论依据。设计:完全随机设计,对照实验研究。地点与材料:7dSD大鼠40只来源于南京医科大学实验动物中心,体质量约14～18g,雌雄不拘,饲养于屏障环境的SPF级实验动物。干预:40只大鼠制成HIBD动物模型,随机分成两组:HIBD模型对照组20只,NGF治疗组在大鼠缺氧缺血后即刻腹腔注射NGF。主要观察指标:观察NGF对HIBD模型的新生大鼠体质量增长情况、死亡率、左右脑质量影响,并用原位缺口末梢标记(insitunickendlabeling,TUNEL)法检测NGF对缺氧缺血后脑细胞凋亡的影响。结果:HIBD模型NGF治疗组体质量增长明显高于对照组(P<0.01);治疗组实验过程中死亡率明显低于对照组;HIBD模型NGF治疗组与对照组健侧(右侧)脑质量相近,但治疗组患侧(左侧)脑质量犤(0.57±0.02)g犦明显重与对照组犤(0.42±0.1)g,P<0.01犦;治疗组左右脑质量无显著差异,而对照组左右脑质量差异有显著性意义(P<0.01);TUNEL染色所见阳性凋亡细胞,其凋亡特征为胞体缩小、核固缩、核碎
BACKGROUND: Apoptosis is a way of neuronal death caused by hypoxic-ischemic brain injury. In vitro experiments show that nervegrowth factor (NGF) deficiency induces neuronal apoptosis. Objective: To investigate the relationship between hypoxic-ischemic braindamage HIBD and apoptosis in neonatal hypoxic-ischemic braindamage and to study the protective effect of NGF on HIBD and to provide a theoretical basis for the treatment of neonatal HIBD with NGF. Design: Completely randomized design, controlled experimental study. Location and Materials: Forty SD rats from 7dSD originated from Laboratory Animal Center of Nanjing Medical University. Their body weight was about 14-18g. SPF animals were housed in barrier environment. Intervention: Forty rats were made into HIBD animal model and randomly divided into two groups: 20 in HIBD model control group and NGF group in NGF treatment group. MAIN OUTCOME MEASURES: The effects of NGF on body weight gain, mortality and left and right brain mass of neonatal rats with HIBD were observed. The effects of NGF on the apoptosis of brain cells after hypoxic-ischemic brain injury were detected by TUNEL influences. Results: The body mass growth of HIBD model group was significantly higher than that of control group (P <0.01). The mortality of experimental group was significantly lower than that of control group in HIBD model group. The NIB group and NGF group (0.57 ± 0.02) g 犦 in the treated group was significantly higher than that in the control group (0.42 ± 0.1) g, P <0.01 犦. There was no significant difference in left and right brain mass between the two groups The difference of brain mass between the left and right groups was significant (P <0.01). The apoptotic cells were positive for TUNEL staining and the apoptotic features were cell shrinkage, nuclear pyknosis and nuclear fragmentation