论文部分内容阅读
目的探索联合激光捕获显微切割(lacer capture microdissection,LCM)与RT-PCR在人口腔鳞癌CDK2AP1基因表达研究中的可行性。方法采用LCM技术从人口腔鳞癌组织冰冻切片中获取肿瘤细胞,提取RNA,并对微量RNA进行纯化。然后用RT-PCR验证总RNA中β-actin及CDK2AP1基因表达水平。结果经LCM后总RNA保持较好完整性;RT-PCR验证β-actin及CDK2AP1基因表达完整。结论通过有效的RNA保护方法,使用LCM技术能成功地获取较为均一的研究目的细胞,RNA完整性较好,能用于人口腔鳞癌CDK2AP1基因研究。
Objective To explore the feasibility of combined laser capture microdissection (LCM) and RT-PCR in the study of CDK2AP1 gene expression in human oral squamous cell carcinoma. Methods Tumor cells were harvested from frozen sections of human oral squamous cell carcinoma tissue by using LCM technique. RNA was extracted and the micro RNA was purified. The expression of β-actin and CDK2AP1 in total RNA was then verified by RT-PCR. Results The total RNA was maintained intact after LCM. The expression of β-actin and CDK2AP1 gene was verified by RT-PCR. Conclusion By using effective RNA protection method, LCM technique can successfully obtain a more uniform target cell. The RNA integrity is better and can be used for the study of CDK2AP1 gene in human oral squamous cell carcinoma.