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目的：观察鬼臼霉素（ＶＭ２６）和手术对胶质瘤细胞分泌β－微量蛋白（β－ｔｐ）水平的影响。方法：用ＥＬＩＳＡ检测不同剂量的ＶＭ２６对体外培养的三种不同类型胶质瘤细胞培养液中β－ｔｐ水平以及测定手术前后胶质瘤病人脑脊液中β－ｔｐ的浓度。结果：①０．３～３０μｇ·ｍｌ－１ＶＭ２６对三种胶质瘤细胞增殖均有抑制作用，培养液中β－ｔｐ的水平亦降低。②少突胶质瘤和星形胶质瘤病人术后脑脊液中β－ｔｐ的水平低于术前，而髓母细胞瘤病人脑脊液中β－ｔｐ的浓度无变化。结论：ＶＭ２６对胶质瘤细胞的增殖有抑制作用；手术切除少突胶质瘤和星形胶质瘤可使病人脑脊液中β－ｔｐ浓度降低。
Objective: To observe the effects of podophyllotoxin (VM26) and surgery on the level of β-microglobulin (β-tp) secreted by glioma cells. Methods: The levels of β-tp in culture media of three different types of glioma cells cultured in vitro with different doses of VM26 were measured by ELISA. The concentration of β-tp in cerebrospinal fluid of patients with glioma before and after operation was measured. Results: ①0.3 ~ 30μg · ml-1VM26 inhibited the proliferation of all three glioma cells, and the level of β-tp in the culture fluid also decreased. ② oligodendrocytes and astrocytoma patients postoperative cerebrospinal fluid β-tp levels lower than preoperative, and medulloblastoma in patients with cerebrospinal fluid β-tp concentration did not change. Conclusion: VM26 can inhibit the proliferation of glioma cells. Surgical resection of oligodendrocytes and astrocytomas can reduce the concentration of β-tp in cerebrospinal fluid of patients.