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目的:采用高效液相色谱(HPLC)法对不同来源的文冠木超微饮片进行指纹图谱研究,为文冠木超微饮片质量控制提供依据。方法:色谱柱:Agilent C18(4.6mm×250mm,5μm)键合硅胶色谱柱;流动相:乙腈-0.4%磷酸(梯度洗脱);流速:1.0mL/min;检测波长:226nm;温度:20℃。结果:以16个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD%均小于2%,10批样品相似度>0.9。并指认了6个主要特征指纹峰,分别为儿茶素、表没食子儿茶素、杨梅树皮素、表儿茶素、2α,3β-双氢杨梅树皮素、双氢槲皮素。其中儿茶素为首次从文冠木药材中分离得到。结论:此方法精密度、稳定性和重复性良好,有助于文冠木超微饮片的质量控制。
OBJECTIVE: To study the fingerprint of Wen Guan Mu ultra-cut from different sources by high-performance liquid chromatography (HPLC), and provide the basis for the quality control of Wen Guan Mu. Method: Column: Agilent C18 (4.6mm × 250mm, 5μm) bonded silica column; mobile phase: acetonitrile-0.4% phosphoric acid (gradient elution); flow rate: 1.0mL / min; detection wavelength: 226nm; temperature: 20 ℃. Results: Using 16 common peaks as evaluation indexes, RSD% of relative peak area and relative retention time of each common peak in precision and repeatability experiments were all less than 2%. The similarity of 10 batches of samples was> 0.9. And identified six main characteristic fingerprint peaks were catechin, epigallocatechin, myricetin, epicatechin, 2α, 3β-dihydromyricetin, dihydroquercetin. Among them, catechin was isolated from Wen Guan Mu medicinal materials for the first time. Conclusion: The precision, stability and reproducibility of this method are good, which is helpful to the quality control of Wen Guan Mu ultrafine cut pieces.