论文部分内容阅读
选用体质量为(5.14±0.09)g的建鲤,采用2因子3水平试验设计,在基础饲料中添加3个DHA水平(0%、0.5%和1.5%)和3个维生素E水平(62.5、125、250 mg/kg)配制9种试验饲料,饲养8周后用嗜水气单胞菌进行攻毒试验,探讨DHA和维生素E对高密度养殖建鲤的免疫力及抗病力的影响。结果表明:饲料中添加维生素E和DHA都可显著提高高密度养殖的建鲤血浆SOD、血浆和脾脏LZM及血浆NO(P<0.05),攻毒存活率亦显著提高(P<0.05)。饲料中添加维生素E可显著降低血浆MDA(P<0.05),维生素E和DHA二者交互作用显著提高脾脏LZM和血浆NO(P<0.05)。在本试验条件下,饲料中添加DHA和维生素E可提高建鲤的抗氧化、免疫力和抗病力,适宜在高密度集约化养殖中作为免疫增强剂适量添加。
The construction carp with the body weight of (5.14 ± 0.09) g was designed with 2 factors and 3 levels. Three DHA levels (0%, 0.5% and 1.5%) and 3 vitamin E levels (62.5, 125, 250 mg / kg) were used to prepare 9 kinds of test diets. After feeding for 8 weeks, the mice were challenged with Aeromonas hydrophila to investigate the effects of DHA and vitamin E on the immunity and disease resistance of Jian carp cultured on high density. The results showed that dietary vitamin E and DHA could significantly increase plasma SOD, plasma and spleen LZM and plasma NO (P <0.05) in high-density culture, and significantly increase the survival rate of challenge (P <0.05). Dietary supplementation with vitamin E significantly reduced plasma MDA (P <0.05). Interaction between vitamin E and DHA significantly increased LZM in spleen and plasma NO (P <0.05). Under the experimental conditions, adding DHA and vitamin E to the feed can improve the anti-oxidation, immunity and disease resistance of Jian carp, and is suitable for adding as an immune enhancer in high-density intensive breeding.