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目的：了解慢性肾衰（CRF）状态及血液透析（HD）环境下患者血清和尿中白细胞介素一8（IL-8）含量的变化规律和IL-8与残余肾功能（RRF）的关系．方法：采用双抗体夹心ELISA法检测25例CRF维持性血透患者和21例CRF非透析患者及26例健康体检者血清和尿中IL-8的含量．结果：CRF患者非HD组和HD组呶清和尿中IL-8的含量均显著高于正常对照组（P＜O．01）;HD组患者透析结束时血清和尿中IL-8的含量明显高于透析前（P＜O．01）。血透结束后48h较血透结束时降低，但仍高于透析前，差别个显著（P＞0．05）;CRF患者非HD组与HD组血泊和尿中IL-8的含量均与Scr无明星相关性计（r＝-0．34．P＞O．05和r＝-0．06．P＞0．05）;HD组中无尿患者（尿量＜400mL／d）血清和尿中IL-8的含量明显高于有尿患者（尿量＞400mL／d）（P＜O．05）．且患者血清和尿中IL-8的含量与尿量成显著负相关（r=一0．467，P＜0．05和r=一0．548．P＜O.05）结论CRF状态和HD环境下都存在严重的免疫炎症反应．且HD对IL-8的产生有明显的诱导作用，此可能为CRF维持性血透患者RRF进一步受损的主要原因之一。
Objective: To investigate the changes of serum and urinary interleukin-8 (IL-8) and the relationship between IL-8 and residual renal function (RRF) in patients with chronic renal failure (CRF) and hemodialysis (HD) . Methods: Serum and urinary IL-8 levels were measured by double antibody sandwich ELISA in 25 patients with CRF-sustaining hemodialysis and 21 patients with CRF non-dialysis and 26 healthy controls. Results: The levels of IL-8 in serum and urine of HD patients were significantly higher than those of normal control group (P <0.01). The levels of IL-8 in serum and urine of HD patients were significantly higher Higher than before dialysis (P <0.01). The level of IL-8 in blood and urine of non-HD group and HD group was significantly higher than that of Scr (P <0.05), and the difference was significant (P> 0.05) There were no star-related indicators (r = -0.34. P> 0.05 and r = -0.06.P> 0.05). Serum and urine in patients without urine (urine output <400 mL / d) The content of IL-8 was significantly higher in patients with urine (urine output> 400 mL / d) (P <0.05). The serum and urinary levels of IL-8 were negatively and significantly correlated with urine volume (r = -0.467, P <0.05 and r = -0.548, P <0.05). Conclusion CRF status and HD Under the environment there is a serious immune inflammation. HD can induce the production of IL-8, which may be one of the main reasons for the further impairment of RRF in CRF-sustaining hemodialysis patients.