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目的:建立RP-HPLC法测定地牡宁神口服液中马钱苷的含量。方法:采用SHIMADZU Shim-pack vp-ODS(250 mm×4.6 mm,5μm)色谱柱,流动相:甲醇-水-甲酸(30:69:1),检测波长:240 nm流速:1.0 ml·min~(-1)。结果:马钱苷的线性范围为0.16—5.56μg,r=0.999 8,平均回收率为101.7%(RSD=1.5%,n=5)。结论:方法简便、准确,可用于地牡宁神口服液的质量控制。
Objective: To establish a RP-HPLC method for determination of loganin in Dimu Ningshen oral liquid. METHODS: A SHIMADZU Shim-pack vp-ODS (250 mm×4.6 mm, 5 μm) column was used. The mobile phase consisted of methanol-water-formic acid (30:69:1) and the detection wavelength was 240 nm. The flow rate was 1.0 ml·min. (-1). Results: The linear range of loganin was 0.16-5.56μg, r=0.999 8, the average recovery was 101.7% (RSD=1.5%, n=5). Conclusion: The method is simple and accurate and can be used for the quality control of Dimu Ningshen oral liquid.