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The synthesis of novel 1-arylsulfonyl -4- (1′-N -2′, 3′, 4′, 6′-tetra-O-acetyl-β-D-glucopyranosyl) thiosemicarbazides via condensation of 2, 3, 4, 6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate with substituted phenylsulfonyl hydrazines was described. Their structures and physical properties were confirmed by 1H NMR, 13C NMR spectra, elemental analysis and MS.