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以1.0×106CFU·m L~(-1)拟态弧菌(Vibrio mimicus)浸浴感染黄颡鱼(Pelteobagrus fulvidraco),于第0、第4、第8、第16、第24、第36、第48、第60、第72小时剖杀取样,研究病理损伤及病原菌的体内动态分布。结果显示,皮肤肌肉、鳃、肠道出现病变最早,其中皮肤肌肉损伤最严重。第16小时表皮变性、坏死,鳃上皮肿胀,肠绒毛水肿;第24~第48小时表皮坏死、脱落,真皮严重出血,肌纤维变性、坏死,鳃出血,上皮局部坏死,肠上皮变性与灶性坏死;第48~第72小时皮肤肌肉坏死更严重,形成溃疡灶,鳃灶性坏死。肾、肝、脾与心36 h后相继出现不同程度的淤血、出血,变性和灶性坏死。q PCR检测发现,第4小时即在鳃、肠、皮肤肌肉检出病菌,细菌含量分别为1.3×102CFU·mg~(-1)、2.6×102CFU·mg~(-1)、4.7×102CFU·mg~(-1);鳃与皮肤肌肉中菌量随时间延长而增加,第72小时皮肤肌肉中菌量达到4.5×107CFU·mg~(-1);第24小时后相继在肾、肝、脾、心检出病菌,菌量介于1.9×10~4.7×102CFU·mg~(-1)之间。结果表明皮肤、鳃和肠道是病菌的入侵位点,并在体内多组织、器官分布。
Pelteobagrus fulvidraco was infested with 1.0 × 106CFU · m L -1 bath of Vibrio mimicus, and was infected with 0, 4, 8, 16, 24, 36, 48, 60, 72 hours sampling, to study the pathological damage and pathogens in vivo dynamic distribution. The results showed that skin lesions, gills, gut appear the earliest lesions, including skin and muscle damage the most serious. Epidermal degeneration and necrosis at 16th hour, gill epithelial swelling and intestinal villus edema; Epidermal necrosis, exfoliation, severe dermal hemorrhage, myofibrosis, necrosis, gill bleeding, epithelial necrosis, intestinal degeneration and focal necrosis from the 24th to the 48th hour ; The 48th to 72nd hours of skin muscle necrosis more serious, the formation of ulcer lesions, gill focal necrosis. Kidney, liver, spleen and heart after 36 h have varying degrees of congestion, bleeding, degeneration and focal necrosis. q PCR detection showed that the bacteria were detected in the gill, intestine and skin muscle at the 4th hour, the bacterial contents were 1.3 × 102CFU · mg -1, 2.6 × 102CFU · mg -1 and 4.7 × 102CFU · The number of bacteria in gill and skin muscle increased with time, the number of bacteria in skin muscle reached 4.5 × 107CFU · mg ~ (-1) after 72 hours. After 24 hours, Spleen, heart detection of bacteria, bacteria range between 1.9 × 10 ~ 4.7 × 102CFU · mg ~ (-1) between. The results show that the skin, gill and intestine are the sites of invasion of germs and are distributed in the body in multiple tissues and organs.