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以 5、10和 2 0 μmol/ L 姜黄素处理 MGC80 - 372 h,细胞存活率随药物浓度及处理时间依赖性下降 ,并呈现典型的凋亡细胞形态 ;以 0、10、2 0及 30 μmol/ L姜黄素处理细胞 2 4 h后 ,2 0 μmol/ L以上的处理组 DNA琼脂糖电泳显示均有典型的凋亡梯形条带 ,同样条件处理后分别对断裂及未断裂 DNA作定量测定 ,计算细胞凋亡率分别为 7.3%、39.2 %、50 .5%和 6 4 .3% ,表明姜黄素具有诱导MGC 80 - 3细胞凋亡的显著作用 .流式细胞术检测显示经 10及 30μmol/ L姜黄素处理的 MGC 80 - 3细胞周期主要阻滞于 G2 / M期 ,提示细胞的 G2 / M期阻滞与姜黄素诱导 MGC80 - 3细胞凋亡有关 .
When MGC80 was treated with 5, 10 and 20 μmol / L curcumin for 372 h, the cell viability decreased with drug concentration and treatment time, and showed typical apoptotic cell morphology; with 0, 10, 20, and 30 μmol After treatment with curcumin for 24 hours, DNA agarose gel electrophoresis showed that the cells had a typical apoptotic trapezoidal band at a concentration of 20 μmol / L or more. After treatment with the same conditions, the fragmented and uncleaved DNA were quantitatively determined. The apoptotic rates were calculated to be 7.3%, 39.2%, 50.5% and 64.3%, respectively, indicating that curcumin has a significant effect on inducing apoptosis of MGC 80-3 cells. Flow cytometry showed that the apoptosis rate was 10 and 30 μmol. The curcumin-treated MGC 80-3 cell cycle was mainly arrested in G2 / M phase, suggesting that the G2 / M phase arrest of cells was related to curcumin-induced apoptosis of MGC80-3 cells.