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目的 :了解对比肾移植受者术后体内淋巴细胞免疫表型的变化情况及其临床意义。方法 :采用多种单克隆抗体及流式细胞仪 ,对肾移植受者术后的淋巴细胞免疫表型进行了化验监测 ,分析了淋巴细胞免疫表型变化与免疫用药及并发症的关系。结果 :移植受者淋巴细胞免疫表型阳性细胞数普遍偏低 (P <0 .0 5 ) ,肾移植后顺利恢复组的淋巴细胞免疫表型阳性细胞与健康对照组无差异 (P >0 .0 5 ) ,肾移植后并发症组受者CD+ 4 、CD+ 8阳性细胞数低于临床正常值 ,NK细胞及CD+ 19阳性细胞数减少 (P <0 .0 5 )。肾移植后急排组的NK细胞及CD+ 19阳性细胞数明显增加 ,CD+ 4 /CD+ 8比值偏高 (P <0 .0 5 )。结论 :淋巴细胞免疫表型可用于评价移植受者的免疫状态 ,临床上根据受者的化验结果综合评价受者的免疫抑制状态 ,并作为调整免疫抑制剂用量的参考指标。
OBJECTIVE: To understand the changes of lymphocyte immune phenotype in postoperative renal transplant recipients and its clinical significance. Methods: Monoclonal antibodies and flow cytometry were used to monitor the immunophenotype of lymphocytes in recipients after renal transplantation. The relationship between immunophenotypic changes of lymphocytes and immune administration and complications was analyzed. Results: The number of lymphocyte immunophenotype positive cells in transplant recipients was generally low (P <0.05). There was no difference in lymphocyte immunophenotypic positive cells between healthy recipients and healthy controls (P> 0.05). The number of CD + 4 and CD + 8 positive cells in the recipients of post-transplant complication group was lower than the normal value, and the number of NK cells and CD + 19 positive cells decreased (P <0.05). The number of NK cells and CD + 19 positive cells in acute rejection group increased significantly after kidney transplantation, and the ratio of CD + 4 / CD + 8 was higher (P <0.05). CONCLUSION: The immunophenotype of lymphocytes can be used to evaluate the immune status of recipients. The immunosuppressive status of recipients can be evaluated clinically based on the results of recipients and can be used as a reference index for adjusting the dosage of immunosuppressants.