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以“冀豆12×黑豆”构建的包含186个家系的F8代重组自交系(RIL)群体为材料,分析16种氨基酸含量,采用性状-标记间单向因素方差分析法(ANOVA)、复合区间作图法(CIM)和完备区间作图法(ICIM)对各氨基酸含量的相关QTL进行检测。结果表明:群体中各性状表型均呈现连续性正态分布并超亲分离。3种方法共检测到谷氨酸、精氨酸、丝氨酸、丙氨酸、苯丙氨酸、异亮氨酸、天冬氨酸和赖氨酸含量相关的14个QTL,分布于B1、D1a、D1b、H、I、J和K连锁群中,贡献率为4.91%~17.02%。其中完备区间作图法检测到的苯丙氨酸含量相关QTL q Phe-20-1贡献率最大为17.02%,增效基因来自于黑豆,并且在该标记区间内利用复合区间作图法同时检测到与天冬氨酸、谷氨酸和赖氨酸含量相关的QTL,分别为q Asp-20、q Glu-20、q Lys-20。位于B1连锁群中精氨酸含量相关的QTL q Arg-11在性状-标记间单向方差分析和ICIM两方法中同时检测到,在ICIM中检测到其贡献率为11.79%,增效基因来自于冀豆12。
A total of 16 Amino acid contents were analyzed in a F8 recombinant inbred line (RIL) population constructed from “Jidou12 × Black Bean ” using 186 lines. The ANOVA method was used to analyze the 16 amino acid contents. , Composite interval mapping (CIM) and complete interval mapping (ICIM) were used to detect the related QTLs for each amino acid content. The results showed that all the phenotypic traits in the population showed a continuous normal distribution and super-pro-segregation. A total of 14 QTLs related to glutamic acid, arginine, serine, alanine, phenylalanine, isoleucine, aspartic acid and lysine were detected by the three methods and distributed in B1, D1a , D1b, H, I, J and K linkage group, the contribution rate was 4.91% ~ 17.02%. Among them, QTL q Phe-20-1 phenotypic phenotype detected by complete interval mapping had the highest contribution rate of 17.02% and the synergistic gene from black bean, and the simultaneous interval detection QTLs related to the content of aspartic acid, glutamic acid and lysine were q Asp-20, q Glu-20 and q Lys-20, respectively. The arginine-related QTL qArg-11 located in the B1 linkage group was detected simultaneously in the trait-marker one-way analysis of variance (ICIM) and in both ICIM methods. The contribution rate of ICR was 11.79% In Jidou 12.