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目的:研究微波辐射对孕鼠脾淋巴细胞中活性氧(ROS)活性和线粒体膜电位(△Ψm)的影响。方法:32只成年雌性Wistar孕鼠随机分为4组,每组8只,其中1组为对照组,3组为实验组;实验组分别采用辐射强度为2.5、5、10 mW/cm2,辐射时间6 min/次,5次/每周,连续辐射4周,对照组不辐射;采用流式细胞术,间接检测细胞内ROS活性和△Ψm。结果:辐射强度为2.5 mW/cm2组细胞内ROS活性和△Ψm与对照组差异无统计学意义(P>0.05);辐射强度5、10mW/cm2组的ROS活性较对照组增加,△Ψm较对照组降低,差异有统计学意义(P<0.05),且呈剂量-反应关系。结论:长期低剂量微波辐射能够诱导孕鼠脾淋巴细胞中ROS活性增加,而△Ψm降低,并且具有一定的剂量效应规律性。
Objective: To study the effect of microwave radiation on reactive oxygen species (ROS) activity and mitochondrial membrane potential (△ Ψm) in pregnant rat spleen lymphocytes. Methods: Thirty-two adult female Wistar pregnant rats were randomly divided into 4 groups with 8 rats in each group. One group was control group and the other three groups were experimental group. The experimental groups were treated with 2.5, 5 and 10 mW / cm2 of radiation intensity Time 6 min / time, 5 times / week, continuous irradiation for 4 weeks, the control group did not radiate; using flow cytometry, indirect detection of intracellular ROS activity and △ Ψm. Results: There was no significant difference in intracellular ROS level and △ Ψm between radiation intensity 2.5 mW / cm2 and control group (P> 0.05). ROS at 5 and 10 mW / cm2 increased compared with control group The control group decreased, the difference was statistically significant (P <0.05), and the dose-response relationship. CONCLUSION: Long-term low-dose microwave irradiation can induce the increase of ROS activity in splenic lymphocytes of pregnant rats, while the decrease of △ ψm, and has some regularity of dose effect.