为观察脑挫伤后NOS1的表达及其与损伤时程的关系 ,采用自制自由落体撞击法致大鼠右顶叶局灶性脑挫伤模型 ,于不同时间段处死大鼠后 ,进行NOS1的免疫组织化学及原位杂交法研究 ,结果发现NOS1及其mRNA表达水平与伤后经过时间有一定相关性 ,即随着伤后经过时间的延长 ,NOS1及其mRNA表达逐渐增加 ,并能较好地鉴别脑挫伤之死前伤和死后伤 ,可作为推断脑挫伤经过时间的参考指标之一。 In order to observe the expression of NOS1 and its relation to the duration of injury after cerebral contusion, the model of focal cerebral contusion in the right parietal lobe was induced by self-made free-fall collision. After being killed at different time points, the NOS1 immunohistochemistry Chemical and in situ hybridization, the results showed that NOS1 mRNA expression level and the passage of time after a certain correlation, that with the passage of time after injury, NOS1 and its mRNA expression increased, and can better identify Pre-injury and post-mortem injury of brain contusion can be used as one of the reference indexes to infer the elapsed time of cerebral contusion.