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目的探讨MMP-9在胃癌中的表达及其与胃癌生物学行为的关系。方法应用免疫组化技术S-P法检测MMP-9基因蛋白在68例胃癌的表达情况,以20例非肿瘤性胃黏膜组织作对照,对随访14个月至13年的35例患者作了生存分析。结果68例胃癌组织MMP-9阳性表达率86.76%(59/68),对照组胃黏膜组织阳性表达率20.00%(4/20)。有淋巴结转移胃癌的MMP-9阳性表达率比无淋巴结转移者高,分别为95.65%、68.18%(P<0.05);侵及黏膜及黏膜下层、肌层和浆膜层胃癌的MMP-9阳性表达率分别为33.33%、91.30%、92.05%(P<0.05)。MMP-9阳性者术后1、3、5、7、10年以上生存率分别为90.00%、56.67%、36.67%、10.00%和3.33%,MMP-9阴性患者术后1、3、5、7、10年以上生存率分别为100%、100%、80.00%、60.00%和40.00%。MMP-9阳性患者术后生存率比MMP-9阴性患者显著降低(P<0.01)。结论MMP-9高表达促进肿瘤转移。MMP-9在胃癌的表达与肿瘤浸润深度及淋巴结转移有关;与患者年龄、性别无关。
Objective To investigate the expression of MMP-9 in gastric cancer and its relationship with the biological behavior of gastric cancer. Methods Immunohistochemical SP method was used to detect the expression of MMP-9 protein in 68 cases of gastric cancer. Twenty cases of non-tumor gastric mucosa were used as controls. Survival analysis was performed on 35 patients who were followed up for 14 months to 13 years . Results The positive expression rate of MMP-9 in 68 cases of gastric carcinoma was 86.76% (59/68), while the positive expression rate of gastric mucosa in control group was 20.00% (4/20). The positive rates of MMP-9 in gastric cancer with lymph node metastasis were higher than those without lymph node metastasis (95.65% and 68.18%, respectively) (P <0.05). MMP-9 positive in gastric mucosa and submucosa The expression rates were 33.33%, 91.30% and 92.05% respectively (P <0.05). The survival rates of MMP-9 positive patients after 1, 3, 5, 7 and 10 years were 90.00%, 56.67%, 36.67%, 10.00% and 3.33%, respectively. The MMP- 7, 10-year survival rates were 100%, 100%, 80.00%, 60.00% and 40.00%. The survival rate of MMP-9-positive patients was significantly lower than that of MMP-9-negative patients (P <0.01). Conclusion High expression of MMP-9 promotes tumor metastasis. The expression of MMP-9 in gastric cancer is related to the depth of tumor invasion and lymph node metastasis, but not to the age and sex of the patients.