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本文利用组织化学方法观察了慢性脑缺血对青、老年大鼠海马ＮＯＳ阳性神经元的影响。慢性脑缺血采用双侧颈总动脉永久性结扎模型。结果：青年缺血１ 月组大鼠海马ＣＡ１、ＣＡ２～３ 和ＤＧ 亚区ＮＯＳ阳性神经元面数密度分别为２９．１±２．３、２３．５±２．１ 和３９．３±２．８，小于对照组相应三个亚区（４９．６±１．３、４９．３±２．１ 和６４．７±２．１），Ｐ＜ ０．０１；青年缺血３ 月组大鼠ＣＡ１、ＣＡ２～３ 和ＤＧ 亚区ＮＯＳ阳性神经元面数密度分别为４０．２±１．６、３９．３±２．５ 和４８．４±１．８，和对照组者相近，但大于缺血１ 月组（Ｐ＜ ０．０１）。老年缺血１ 月组大鼠海马各区ＮＯＳ阳性神经元面数密度分别为３９．６±１．５、３５．６±２．１ 和５４．７±２．５，小于老年对照组相应三个亚区（５５．８±１．７、５１．３±１．７ 和６４．９±１．９），Ｐ＜ ０．０１；老年缺血３ 月组大鼠各亚区ＮＯＳ阳性神经元面数密度分别为４３．１±２．４、３８．７±３．４ 和５４．７±３．２，仍然小于对照组，Ｐ＜ ０．０１。结果提示：慢性脑缺血可以影响大鼠海马ＮＯＳ阳性神经元，但青年和老年大鼠海马ＮＯＳ神经元对慢性脑缺血损伤的易感性和反应性不同。
In this paper, histochemistry was used to observe the effects of chronic cerebral ischemia on NOS positive neurons in hippocampus of young and old rats. Chronic cerebral ischemia using bilateral common carotid artery ligation model. Results: The number density of NOS positive neurons in hippocampal CA1, CA2 ~ 3 and DG subfields in young group were 29.1 ± 2.3, 23.5 ± 2.1 and 39.3 ± 2 .8, less than the corresponding three sub-regions of the control group (49.6 ± 1.3, 49.3 ± 2.1 and 64.7 ± 2.1), P <0.01; The numbers of NOS positive neurons in rat CA1, CA2 ~ 3 and DG subfields were 40.2 ± 1.6,39.3 ± 2.5 and 48.4 ± 1.8, respectively, which were similar to those in the control group Greater than the ischemic January group (P <0.01). The number density of NOS positive neurons in the hippocampus of the aged group was 39.6 ± 1.5,35.6 ± 2.1 and 54.7 ± 2.5, respectively, which was less than that of the control group Subregion (55.8 ± 1.7,51.3 ± 1.7 and 64.9 ± 1.9), P <0.01; NOS positive neurons The number densities were 43.1 ± 2.4,38.7 ± 3.4 and 54.7 ± 3.2, respectively, which were still less than those in the control group (P <0.01). The results suggest that chronic cerebral ischemia can affect NOS positive neurons in hippocampus of rats, but the hippocampal NOS neurons in young and old rats have different susceptibility and reactivity to chronic cerebral ischemia.