论文部分内容阅读
本文首次用合成的羧甲基化的以N，N’-甲叉双丙烯酰胺交联的烯丙基葡聚糖（简称CM—CADB）凝胶生化树脂为新型载体，研究了谷氨酸脱羧酸在CM—CADB树脂上的固定化与环境的依赖关系。确定了酶固定化最佳条件：缓冲体系为pH4．4，0．10mol／L磷酸氢二钠-0．05mol／L柠檬酸，载体为交联度35％，交换容量3．0meq／g的CM－CADB凝胶树脂，吸附平衡时间4h以上，酶用量为30．0mg／gdryresin，固定化环境温度控制在40℃。从理论上分析了上述结果。
In this paper, for the first time, a new carrageenan-based biocompatible carboxymethylated allyl dextran (CM-CADB) gel resin crosslinked with N, N’-methylenebisacrylamide was used to study the effect of glutamate decarboxylation Acidity in CM-CADB resin immobilization and environmental dependence. The optimum conditions of enzyme immobilization were determined: the buffer system was pH4.4, 0.10mol / L disodium hydrogen phosphate -0.05mol / L citric acid, the carrier was 35% crosslinking degree, the exchange capacity was 3.0meq / g CM-CADB gel resin, adsorption equilibrium time more than 4h, the amount of enzyme 30.0mg / gdryresin, immobilized environment temperature controlled at 40 ℃. The above results are theoretically analyzed.