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This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body (PLB) formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88 μmol L-1 N6-benzyladenine (BA). The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L-1 2,4-D and 4.44 μmol L-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole of A. andraeanum.
This tissue culture technique, the conditions for callus induction, protocorm-like body (PLB) formation and Plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulators, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L-1 2,4-dichlorophenoxyacetic acid 2,4-D) and 8.88 μmol L-1 N6-benzyladenine (BA). The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and maintained by subculturing on 1/2 MS medium containing 0.90 μmol L-1 2,4-D and 4.44 μmol L-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L -1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole of A. andraeanum.