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目的:建立小鼠淋巴瘤的原位脑肿瘤模型。方法:鼠源性B淋巴瘤细胞株A20,调整浓度到1×108 mL-1。在立体定向仪下将A20淋巴瘤细胞0.5μL植入BALB/c小鼠大脑中,每日观察,隔2d称体质量。3周后处死,制作石蜡切片做免疫组化及病理分析。结果:免疫组化及病理显示成功种植淋巴瘤,种植后小鼠体质量变化情况及存活时间恒定有规律。该方法建立的脑淋巴瘤动物模型在组织病理学上接近人脑淋巴瘤,且颅内生长稳定,成功率为80%。未见颅外转移病灶,实验周期短,重复性较好。结论:植入小鼠同源的B系淋巴瘤细胞株A20,可在小鼠大脑中形成原位肿瘤,其形态及恶性程度符合脑淋巴瘤模型的要求,生长特性及病理特征与人脑淋巴瘤相似,可作为临床淋巴瘤基础研究的理想模型。
Objective: To establish an in situ brain tumor model of mouse lymphoma. Methods: Murine B-cell lymphoma cell line A20 was adjusted to a concentration of 1 × 108 mL-1. 0.5μL A20 lymphoma cells were implanted into the brain of BALB / c mice under stereotaxic instrument. The body weight was measured every 2 days. After 3 weeks, sacrifice, making paraffin sections for immunohistochemistry and pathological analysis. Results: Lymphoma was successfully established by immunohistochemistry and pathology. The body weight and survival time of mice after planting were constant and regular. The animal model of brain lymphoma established by this method is histopathologically close to human brain lymphoma with stable intracranial growth with a success rate of 80%. No extracranial metastases, the experimental period is short, good repeatability. CONCLUSION: Mouse-derived homologous B lymphoma cell line A20 can form in situ tumor in mouse brain. Its morphology and malignancy are in accordance with the requirements of brain lymphoma model. The growth characteristics and pathological features are similar to those of human brain lymph Tumor similar, can be used as an ideal model for basic research in clinical lymphoma.