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目的 探讨电磁辐射损伤效应机制中儿茶酚胺的作用。方法 采用高效液相色谱法检测电磁辐射后 0、8、2 4、4 8h大鼠血清、海马组织去甲肾上腺素 (NE)、肾上腺素 (E)、多巴胺 (DA)的含量变化 ,并且观察了铝箔全身屏蔽和躯干屏蔽两种防护措施对儿茶酚胺的影响。结果 无屏蔽大鼠辐射后血清和海马组织中的儿茶酚胺即刻均明显升高 ,差异有显著性 (P <0 .0 1或P <0 .0 5 ) ,8h有所下降 ,2 4h再次上升 ,4 8h海马组织NE、DA仍处于较高水平 (P <0 .0 1) ,血清中NE、DA接近对照组 ;全身屏蔽辐射后儿茶酚胺含量无明显变化 ;躯干屏蔽组 6 5W/cm2 辐射后只有 2 4h时相点明显升高(P <0 .0 5 ) ;躯干屏蔽组 12 9W/cm2 辐射后儿茶酚胺含量变化趋势与未屏蔽组类似 ,且其升高幅度接近甚至超过未屏蔽组。结论 儿茶酚胺可能参与了电磁辐射对动物中枢神经系统及心血管系统的损伤 ,且中枢神经系统损伤效应持续时间长于外周心血管系统。全身屏蔽的防护效果最好 ,在对电磁辐射采取防护措施中 ,最首要是重视大脑的防护
Objective To investigate the role of catecholamines in the mechanism of electromagnetic radiation injury. Methods The contents of norepinephrine (NE), epinephrine (E) and dopamine (DA) in serum and hippocampus of rats at 0, 8, 2, 4 and 8 h after EMF were measured by high performance liquid chromatography The whole body foil shield and torso shield two protective measures on the impact of catecholamines. Results The levels of catecholamines in the serum and hippocampus of the unshielded rats were significantly increased immediately after radiation (P <0.01 or P <0.05), decreased at 8 h and then increased again at 24 h, NE and DA in the hippocampus were still at a high level (P <0.01) at 48th hour, while NE and DA in the serum were close to the control group. There was no significant change in the content of catecholamine after the whole body shielding radiation. (P <0.05). The trend of catecholamine content in trunk shield group after 12 9 W / cm2 irradiation was similar to that of unshielded group, and its increasing range was close to or even exceeded that of unshielded group. Conclusion Catecholamines may be involved in the damage of central nervous system and cardiovascular system by electromagnetic radiation, and the damage of central nervous system is longer than that of peripheral cardiovascular system. Body shielding the best protective effect, in the protective measures against electromagnetic radiation, the most important thing is to pay attention to the protection of the brain