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目的:观察苦参素注射液对实验性自身免疫性脑脊髓炎(EAE)大鼠一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)表达的影响,探讨苦参素苦参素对EAE大鼠的防治机制。方法:采用豚鼠脊髓匀浆+完全弗氏佐剂复制EAE模型,动物随机分为正常组,模型组,苦参素高、低剂量组(150,200 mg/kg),连续给药16天,并每天对各组大鼠进行神经功能学评分、苏木素-伊红(HE)和变色酸2R-亮绿(C-2R-BG)染色观察病理改变、硝酸还原酶法测定EAE大鼠血清中NO含量的变化、免疫组织化学法和RT-PCR法分别检测脊髓中iNOS及其mRNA的表达。结果:苦参素注射液150mg/kg能明显降低EAE大鼠的神经功能学评分,减轻模型组大鼠中枢神经系统炎症浸润和脱髓鞘,减少血清中NO的含量,抑制脊髓中iNOS及其mRNA的表达;与低剂量组相比,高剂量组(200mg/kg)对NO/iNOS系统的表达并没有显著差异。结论:苦参素注射液对EAE大鼠具有防治作用,其机制可能与抑制大鼠血清和脊髓中NO和iNOS的表达有关。
Objective: To observe the effect of oxymatrine injection on nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression in experimental autoimmune encephalomyelitis (EAE) rats, Prevention and treatment of EAE rats. Methods: The guinea pig spinal cord homogenate and complete Freund’s adjuvant were used to replicate the EAE model. The animals were randomly divided into normal group, model group and high and low dose oxymatrine (150 and 200 mg / kg) Neurological scores, hematoxylin-eosin (HE) and chromotachic acid 2R-Brilliant Green (C-2R-BG) staining were used to observe the pathological changes in each group. Nitric acid reductase was used to determine the NO content in the serum of EAE rats The expression of iNOS and its mRNA in the spinal cord were detected by immunohistochemistry and RT-PCR. Results: Oxymatrine injection 150mg / kg can significantly reduce the EAE rats neurological score, reduce the model group of central nervous system inflammatory infiltration and demyelination, reduce the content of NO in serum, inhibit spinal cord iNOS and its Compared with the low dose group, the high dose group (200mg / kg) had no significant difference in NO / iNOS expression. Conclusion: Oxymatrine injection has preventive and therapeutic effects on EAE rats, which may be related to the inhibition of NO and iNOS expression in serum and spinal cord of rats.