论文部分内容阅读
目的:探讨不同归经芳香药藿香、苍术在体外甲型H1N1(猪流感)病毒感染时,对其免疫细胞的免疫信号相关通路调节的差异,从而揭示藿香、苍术抵抗甲型H1N1病毒的免疫作用机制和原理。方法:利用酶联免疫吸附实验(ELISA)检测RIG-1样解旋酶(RLH)途径相关的免疫细胞因子的表达,对比肺经芳香药藿香与非肺经芳香药苍术两者在甲型H1N1病毒的相关免疫通路的差异。结果:霍香提取部位A组各含量与模型组比较,霍香提取部位B组TNF-Q、IFN-γ含量与模型组比较,差异都具有统计学意义(P<0.05);苍术提取部位A组IL-10与模型组比较,苍术提取部位B组IFN-γ含量与模型组比较,差异都具有统计学意义(P<0.05)。结论:藿香对甲型流感病毒感染的小鼠肺炎治疗效果,与其提高血清中IFN-γ、IL-10的表达有关,藿香比苍术对甲型H1N1流感相关的免疫调节作用更为显著。
OBJECTIVE: To investigate the differences in the regulation of immune signal-related pathways of different vaccinated aromatic herbs Agastache rugosae and Atractylodes macrocephala against the influenza A (H1N1) virus infection in vitro so as to reveal the resistance of Agastache rugosa and Atractylodes Immune mechanism and principles of action. METHODS: The expression of immunocytokines related to the RIG-1-like helicase (RLH) pathway was detected by enzyme-linked immunosorbent assay (ELISA) Differences in related immune pathways of the H1N1 virus. Results: Compared with the model group, the contents of TNF-Q and IFN-γin B group of Huoxiang extraction group were significantly lower than those in model group (P <0.05) Compared with the model group, the difference of IFN-γ content in the herb extract group B group and the model group was statistically significant (P <0.05). Conclusions: Agastache rugosa extract is effective in treating pneumonia in mice infected with influenza A virus, which is related to increasing the expression of IFN-γ and IL-10 in serum. Agkistrodon acutus is more effective than Atractylodes macrocephala in regulating influenza A (H1N1) -like influenza.