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目的 :检测脑血栓 (CT)和急性心肌梗死 (AMI)患者活化蛋白C(APC)抵抗 (APCR)及APC裂解凝血因子V (FV )和凝血因子VIII(FVIII)肽链的基因位点突变。方法 :用单链构象多态性分析 (SSCP)方法 ,检测 10 2例CT、46例AMI和 10 5例健康人的APC裂解FV肽链 (Arg3 0 6、Arg 5 0 6、Arg 679和结合部位 1865～ 1875 )、裂解FVIII肽链 (Arg3 3 6、Arg 5 62和结合部位 2 0 0 5～ 2 0 18)的基因位点。结果 :对照组和患者组均未检出FV的突变基因。结论 :中国人动脉血栓性疾病患者无APC裂解FV和FVIII肽链的基因突变 ,患者的APC敏感性的降低可能由其他因素所致
OBJECTIVE: To detect APCR and APC gene mutations in patients with cerebral thrombosis (CT) and acute myocardial infarction (AMI) and the APC lytic factor V (FV) and coagulation factor VIII (FVIII) peptide. Methods: Single strand conformation polymorphism (SSCP) was used to detect APC cleavage of FV peptide chains (Arg3 0 6, Arg 5 0 6, Arg 679 and binding Site 1865-1875) cleaves the gene site of the FVIII peptide chain (Arg336, Arg562 and binding sites 20000 to 20818). Results: No mutation gene of FV was detected in control group and patient group. CONCLUSIONS: In Chinese patients with arterial thrombotic disease, there is no genetic mutation of APC-cleaved FV and FVIII peptide chains. The reduction of APC sensitivity may be caused by other factors