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目的观察野百合碱葛根素联合用药后大鼠肺组织差异蛋白质组学的变化并探讨葛根素抑制肺动脉高压、肺血管重建的机制。方法随机将SD大鼠分为5组,正常组、溶剂组、2个时间模型组、实验组,每组10只。溶剂组,腹腔注射乙醇与0.9%氯化钠混合溶液(50 mg·kg-1体重)每晨1次,饲养3周。模型组,腹腔注射野百合碱(50 mg·kg-1体重)1次,分别饲养2,3周。实验组,注射野百合碱溶液后,每晨腹腔注射葛根素液(40 mg·kg-1体重)1次,饲养3周。制备模型并确定其制备成功,对各组肺组织的总蛋白质进行双向电泳,质谱鉴定差异蛋白质,并进行数据库检索与分析。结果葛根素可抑制野百合碱引起的肺动脉高压、肺血管重建与右心室肥大。延伸因子2在2周,3周野百合碱组中表达依次递增,与正常组有显著差异。在葛根素组呈下调趋势,与野百合碱组差别显著。结论延伸因子2可能与葛根素抑制肺动脉高压的机制相关。
OBJECTIVE: To observe the differential proteomics changes of rat lung tissue after the combination of wild lily and puerarin, and to explore the mechanism of puerarin inhibiting pulmonary hypertension and pulmonary vascular remodeling. Methods SD rats were randomly divided into 5 groups, normal group, solvent group, 2 time model group, experimental group, 10 rats in each group. Solvent group, intraperitoneal injection of ethanol and 0.9% sodium chloride mixed solution (50 mg · kg-1 body weight) every morning 1, feeding 3 weeks. Model group, intraperitoneal injection of monocrotaline (50 mg · kg-1 body weight) 1, respectively, feeding 2, 3 weeks. Experimental group, intraperitoneal injection of puerarin solution (40 mg · kg-1 body weight) once a week for 3 weeks after injection of monocrotaline solution. The model was prepared and its preparation was successful. The total protein in each group was subjected to two-dimensional electrophoresis. The differential proteins were identified by mass spectrometry. The database was searched and analyzed. Results Puerarin inhibited monocrotaline-induced pulmonary hypertension, pulmonary vascular remodeling and right ventricular hypertrophy. The expression of elongation factor 2 in the two weeks and three weeks in the monocrotaline group increased in sequence with significant difference from the normal group. The puerarin group showed a downward trend, significant difference with the monocrotaline group. Conclusions The elongation factor 2 may be related to the mechanism of puerarin inhibiting pulmonary hypertension.