论文部分内容阅读
目的 验证神经细胞和胶质细胞 (NG)对胰岛细胞的营养和生长促进作用。方法 采用SD大鼠NG与SD大鼠及新生猪胰岛样细胞团 (ICCs) ,进行同种属和异种属细胞间的共同培养。用倒置显微镜、透射和扫描电镜观察两种细胞的生长状况 ,MTT法测定细胞的活力 ,放免法和比色法测定胰岛素和淀粉酶的含量 ,免疫组化鉴定共同培养的细胞。结果 共同培养能显著延长同种属及异种属胰岛细胞的生存期 (>1月 ) ,增加ICCs的活力和胰岛素的分泌 (P <0 0 5 ) ,减少胰淀粉酶的分泌 (P <0 0 5 )。抑制成纤维细胞的过度增生。结论 共同培养能为胰岛细胞的生长提供一个优良的微环境。这为脑内胰岛移植提供了一个新依据 ;为胰岛细胞的体外保存提供了一个新方法。在移植前与NG共同培养以消除外分泌部细胞的影响 ,也是一个好策略。
Objective To verify the effects of nerve cells and glial cells (NG) on the nutrition and growth of islet cells. Methods SD rat NG and SD rats and neonatal porcine islet-like cell clusters (ICCs) were used to co-culture the same species and xenogeneic cells. The growth of the two cells was observed by inverted microscope, transmission electron microscope and scanning electron microscope. The viability of cells was determined by MTT assay. The levels of insulin and amylase were determined by radioimmunoassay and colorimetric assay. The co-cultured cells were identified by immunohistochemistry. Results Co-culture could significantly prolong the survival time of islet and xenogeneic islet cells (> 1 month), increase the activity of ICCs and insulin secretion (P <0.05), and decrease the secretion of pancreatic amylase (P <0 0 5). Inhibits fibroblast hyperplasia. Conclusion Co-culture can provide an excellent microenvironment for the growth of islet cells. This provides a new basis for islet transplantation in the brain and provides a new method for the islet cell preservation in vitro. It is also a good strategy to co-culture with NG before transplantation to eliminate the effects of exocrine cells.